Digital Polarimeter AUTOPOL® V Plus

The AUTOPOL® V Plus Digital Polarimeter has the validation tools and features to satisfy today’s global pharmaceutical companies, including: instrument level 21CFR Part 11 compliance, NIST traceable calibration standards and a global list of installations. Rudolph Research has thousands of satisfied customers who praise the Autopol® V Plus’s quality, accuracy and reproducibility.


Trouble viewing this video? Click Here

AUTOPOL® V Plus Polarimeter Standard Features and Accessories

  • TempTrol™ — Electronic cooling & heating from 15°-35°C
  • Six Standard Wavelengths: 365nm, 405nm, 436nm, 546nm, 589nm and 633nm
  • 21CFR Part 11 Compliance: Electronic Signature and secure local electronic data storage
  • Domestic 3-year warranty and 20-year support guarantee
  • International warranty varies by country
  • Standard Accessories: TempTrol™ Ceramic Quartz, NIST Traceable Quartz Standard (three rotation), TempTrol™ 100mm Polarimeter Cell, TempTrol™, Temperature Validation Cell, Built-In Sample Measurement Probe

The AUTOPOL® V Plus Advantage

  • NEDOX®: Silco Steel coating on the polarimeter chamber temperature transfer surface and a Ceramic Quartz measurement cell with NIST certificate certifying optical path length.
  • To meet the FDA’s latest position on measurement bracketing, the Autopol® V Plus comes with a NIST traceable quartz plate with three rotations: -10.7290°, +11.5130°, and combined +0.7840°. Each rotation has a NIST certificate.
  • Windows based navigation architecture is so intuitive that most operators will never read the manual. But should you wish to reference the manual, it is stored right on the Autopol® V Plus’ internal memory.
  • Available with automated sampling.
  • Four USB ports allow quick and easy connection to a mouse, keyboard, printer, bar code scanner, or memory stick. One USB port is located on the front of the unit (shown above) and three USB ports are located on the back of the unit (shown below).

    Network and Printer Communications Simplified

  • Ethernet Port for Network Cable Connection
  • Internet access allowing connection to Rudolph’s service department for remote testing and diagnostics
  • Connect to any Windows® based printer via USB or direct to the server via Windows® Print Library
  • Save measurement data direct to your Network/Server/USB Drive

2 Quartz Plates are stored within the instrument, ready for automatic calibration. Rudolph is the only Polarimeter manufacturer in the world producing certified 3 rotation Quartz Calibration Standards accredited by NVLAP and traceable to NIST, with an accuracy of ± 0.002 degrees Optical Rotation.

Rudolph strongly encourages Quartz Calibration Standards with 3 rotational values above, below and near the sample under test to comply with FDA rulings related to “Sample Bracketing” and EP’s most recent calibration guidelines on linearity. Each Quartz Standard is certified by an accredited independent body (NVLAP ) and Traceable to NIST.

Each Quartz plate is positioned for calibration by the instrument right from the touch screen. Calibration is thus fully automated and requires no handling of the Quartz Control Plate.


Patented TempTrol™ Technology Eliminates the Need for a Water Bath. Here is How the TempTrol™ System Works:

Temperature is selected via touch screen. Temperature selection of 25°C shown above.

Ceramic Quartz TempTrol™ cell with NEDOX™ mating heating & cooling transfer surface manufactured with acid resistance materials:  NEDOX™ and Peek™ below.

TempTrol™ heating and cooling transfer surface

Temperature  Measurement Port

For improved temperature performance or when working with acids (HCl) use the measurement port in the body of the cell. This also allows the inlet and outlet ports to be capped to avoid evaporation, fumes and spillage.

NEDOX® Coated Temperature measurement probe

Place the TempTrol™ cell in your TempTrol™ equipped Autopol® sample chamber to measure to within ±0.2°C of the USP, EP, JP or BP specified temperature (normally 20°C or 25°C ±0.5°C).

Place the TempTrol™ cell in your TempTrol™ equipped Autopol® polarimeter sample chamber to measure within ±0.2°C of the USP, EP, JP, or BP specified temperature (normally 20°C or 25°C ±0.5°C). For improved temperature performance or when working with acids (HCl) use the measurement port in the body of the cell. This also allows the inlet and outlet ports to be capped to avoid evaporation, fumes and spillage.

Ceramic Quartz Cells

• Rudolph supplies Ceramic Quartz Cells as standard on Autopol V, V PLUS and VI polarimeters and guaranteed against breakage or damage caused by acids, for the life of the instrument.

• Cells are resistant to 6 Molar HCL – Ceramic Quartz Cells are impervious to acids and transfer heat quickly and evenly.

• Rudolph’s exclusive, patented TempTrolTM cells use an acid resistant NEDOXTM coated transfer surface to mate with the NEDOXTM coated TempTrolTM active heating and cooling transfer surface.

• Rudolph Polarimeter cells are easy to handle, lightweight, easy to fill and easy to clean. Cell ends are removable for thorough cleaning.

• Rudolph Cells are light and have a large thermal transfer surface so they come to temperature quickly and evenly across the cell.

• Cell apertures are smaller than the internal bore diameter, therefore, small air bubbles do not impact sample measurement.

Rudolph provides a temperature validation cell with every TempTrol™ system. The temperature validation cell along with optional NIST traceable Thermo Probe is designed to validate the temperature control performance of the polarimeter and cell to ±0.2°C.

The Rudolph AutoFillTM

Filling a Polarimeter Cell has never been easier.

Rudolph Research Analytical developed AutoFillTM, Patent No. 10,101,353, a simple and easy way to allow
fast and reliable polarimeter cell loading. Polarimeter sample cells are filled without bubbles, using Rudolph
propriatary BubbleInspectTM technology, sample vapors are contained, and cleaning and drying is automated.
Manual cell handling, cleaning and loading is eliminated.

The AutoFillTM (Patent No. 10,101,353) is available as an option with
BubbleInspectTM on the Autopol V Plus and Autopol VI Polarimeters.

Engineered for perfect fills every time.

Operation is as simple as opening the AutoFillTM lid , pouring in your
sample, closing the lid and pressing start. The sample will be advanced into
the Polarimeter cell and stopped once full. The cell is now loaded and the
measurement will automatically begin after BubbleInspectTM approves the measurement.
When the measurement is complete the operator is prompted to open the AutoFillTM
chamber and add a solvent. The AutoFillTM lid is closed and the solution is flushed
through the cell to waste. Air drying will automatically begin and end.
You are now ready for your next measurement.

AutoFillTM reduces sample loading and cleaning time

• No need to inject samples with syringes. Samples are easily poured
   in the AutoFillTM sample well.

• Eliminate contact with acids and bases.

• Air dry is run for the correct amount of time to fully dry the cell.

• Cleaning is made effortless.

• A perfect cell load is guaranteed every time with Rudolph’s exclusive
   Optical Rotation Homogeneity Inspection Solution.

If Acids and Open Samples are Allowed in the Work Area

• Sample is easily poured into the large AutoFillTM well.

• All wetted parts are made to stand up against the most aggressive
   samples including 6 Molar HCl

• All Materials are Teflon, Peek, and Kalrez®.

• You may choose to clean with a single solvent or a
   combination of solvents.

A Fully Digital Polarimeter with an Intuitive Windows® Embedded Based Interface

  • 32 gigabytes of internal memory allow almost unlimited capacity for saving measurement data. The AUTOPOL® V PLUS is network ready and data may also be saved directly to your server or to any directory desired.
  • Internet access is possible directly from the AUTOPOL® V PLUS’s touch screen. This feature allows real time contact with Rudolph Research Analytical’s Service and Technical Support Team who can access your instrument remotely to assist in Window navigation, method setup and troubleshooting.
  • Windows based navigation architecture is so intuitive that most operators will never read the manual. But should you wish to reference the manual, it is stored right on the AUTOPOL® V PLUS’s internal memory.
  • Four USB ports allow quick and easy connection to a mouse, keyboard, printer, bar code scanner, or memory stick. One USB port is located on the front of the unit and three USB ports are located on the back of the unit.

Flexible Method Management

Install your own measurement methods for immediate selection of the correct method to match your most common tests. Setting up your own custom methods is as simple as filling out a few screens like the one shown to the right.

Full 21CFR Part 11 Instrument Level Compliance

The AUTOPOL VI’s 21CFR Part 11 software module is easily enabled through the user friendly touch screen. This module gives you full compliance with:

  • Electronic signature
  • Access levels
  • Internal write protected storage
  • Unique passwords
  • Write protected documents sent directly to server
  • Audit trail


21CFR11 mode is enabled via the Set 21CFR11 button. After clicking the Enable 21CFR11 button, the user must also select to either Enable Operator, Enable Review or both.

Add/Edit Users allows the creation of new 21CFR11 users, the ability to edit an existing user, and the ability to lock and unlock accounts.


Groups are used to determine the access permissions of the 21CFR11 users. When a group is added or edited, the administrator decides what menus the group will have access to in the Setup menu and sub-menus, and the 21CFR11 Administration Options menu and sub-menus.


Password Settings control the password policy used by the Autopol to authenticate users and whether the screen lock function is active and how long it takes for the screen lock to turn on.


Terminology allows the Administrator to customize the language of the Accept/Reject language that appears in the measurement report reviewed by a reviewer.


The Format Printing/Output menu lets the user control what information will be contained in the report, if the report will contain a logo image, the page orientation and margin settings and if the user will be prompted for a lot identifier and sample identifier when the measurements are being performed.


Print your customized Certificate  of Analysis including your company logo directly from the Autopol VI® touch screen

Rudolph Research Analytical — still protecting your investment after 30 years

Today our Service Technicians have access to state of the art diagnostic tools and have a depth of knowledge that often lets them solve problems right over the phone. Our customers really appreciate the same day phone call by a real technical service person who wants to solve their problem. Our customers know that they can expect this support for many years to come as Rudolph is still repairing instruments which were built in the 70’s. We manufacture Digital Polarimeters and back them with a 20 year commitment to service them!


All Rudolph Instruments carry our exclusive 20 Commitment to service your instrument.

Rudolph Research Analytical offers a comprehensive range of Preventive Maintenance and Service Programs. Rudolph has demonstrated a commitment to its customers by keeping installed instruments operational, not only for 20 years, but in some cases for over 40 years. This long-term commitment to keeping our instruments running makes the cost of ownership, over the life of a Rudolph Instrument, one of the lowest in the laboratory market segment. At the date this document was published, there is no other laboratory instrument manufacturer guaranteeing service and technical support for 20 years. View the entire 20 Service Guarantee. 


Autopol V Plus – Specifications

AUTOPOL® V PLUS Specifications
Measuring Mode

Optical Rotation
Specific Rotation
Specific Rotation Plus Concentration
User Defined Scale
Sugar Degrees

Measuring Scale

Degrees Arc, % Concentration

Measuring Range

± 89° Arc Optical Rotation
± 999.99° Arc Specific Rotation
0-99.9% Concentration


0.001° Arc Optical Rotation
0.001% Concentration
0.001° Specific Rotation


0.002° Arc

Standard Model Accuracy

0.002° up to 1°, 0.2% up to 5°, 0.01° above 5°

AP Model Accuracy and Resolution (Autopol V Plus Single)

Accuracy: ±0.002° Arc over ±89.9° Arc
Selectable Resolution 0.01°, 0.001°, 0.0001° Arc Optical Rotation

AP Model Accuracy and Resolution (Autopol V Plus Six Wavelength)

Accuracy (546nm and 589nm): ±0.002° Arc over ±89.9° Arc
Selectable Resolution 0.01°, 0.001°, 0.0001° Arc Optical Rotation


Glan Thomson calcite quartz

Optical Wavelengths

365nm, 405nm, 436nm, 546nm, 589nm, 633nm
(other wavelengths available)

Wavelength Selection

Touch Screen Selectable: 6 Standard

TempTrol™ Range

Automatic Electronic Heating & Cooling 15°-35°C

TempTrol™ Accuracy

± 0.2°C

Temp. Probe Range


Temp. Probe Accuracy

± 0.1°C

Acid Resistance

Hastelloy™ measurement cell and Silco Steel™ sample chamber (standard)

Measurement Time

4°/sec slewing rate & 5 sec nominal settling time

Light Source

Tungsten-halogen 6V, 20W, avg. 2,000 hr life

Sample Chamber

Accepts sample tubes up to 200mm

Data Storage

32 GB Non-removable Compact Flash

Communication Interface

3 USB Ports, 2 RS232 Ports, Ethernet Port for Network Connection


Automatic calibration via touch screeen

Operating System

Windows® Embedded


10.4 inch diagonal, 800x600 pixels, color, Flat Panel Monitor with Resistance Touch Screen Interface, 200 nits brightness, gasketted for spill protection

User Interface


Automatic Sensitivity Control

Measures samples with transmittance as low as 0.01% (up to O.D. 4.0)

Input Power

85-260 VAC; 48-62 Hz,
150-200 Watts consumption

Operating Dimensions

32"W x 11.5"H x 19.5"D
813mm W x 292mm H x 457mm D

Shipping Dimensions

43"W x 28"H x 23"D
1411mm W x 919mm H x 722mm D

Operating Weight

90 lbs (41 kg)

Shipping Weight

140 lbs (64 kg)